2-Nitrophenyl β-D-galactopyranoside
SIGMA/N1127 - ≥98% (enzymatic)
Synonym: ONPG ; o-Nitrophenyl β-D-galactopyranoside; ONPG
CAS Number: 369-07-3
Empirical Formula (Hill Notation): C12H15NO8
Molecular Weight: 301.25
EC Number: 206-716-1
MDL Number: MFCD00063255
Linear Formula: C12H15NO8
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N1127-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N1127-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N1127-500MG
| assay | ≥98% (enzymatic) |
| form | powder |
| grade | Molecular Biology |
| InChI | 1S/C12H15NO8/c14-5-8-9(15 |
| InChI key | KUWPCJHYPSUOFW-YBXAARCKSA |
| Quality Level | 200  |
| SMILES string | OC[C@H]1O[C@@H](Oc2ccccc2 |
| storage temp. | −20°C |
| Application: | 2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase. |
| Biochem/physiol Actions: | β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period. |
| General description: | Chromogenic substrate for β-galactosidase |
| General description: | ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase. |
| Packaging: | 1, 5, 25 g in poly bottle |
| Packaging: | 250 mg in glass bottle |
| Packaging: | 500 mg in poly bottle |
| Preparation Note: | A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (enzymatic) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |