NGF-β human
SIGMA/N1408 - from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
Synonym: HSAN5; HSAN5; NGF-β human; NGFB; NGFB; hNGFβ; hNGFβ
MDL Number: MFCD00164982
Product Type: Chemical
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| assay | >97% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | <1 EU/μg |
| packaging | pkg of 0.1 mg |
| pkg of 5X0.1 mg | |
| potency | 0.15-3.0 ng/mL ED50/EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in NSO cells |
| solubility | water: soluble 0.1 g/L at 20 °C |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01138  |
| Analysis Note: | The biological activity is measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1. |
| Application: | NGF-β human has been used: • in protein pin arrays • to induce U87-MG (glioblastoma cell line) cell proliferation • in drug release kinetics |
| Biochem/physiol Actions: | Nerve growth factor β (NGFB) acts as a key mediator in neuronal and non-neuronal development, differentiation, and survival. Polymorphism of NGFB gene leads to hereditary sensory and autonomic neuropathies. Charcot arthropathy and thermal insensitivity are few of the related symptoms. NGFβ is known to promote cancer cell proliferation and metastasis, also mediates angiogenesis. It facilitates the development of various cancer including, oral, breast, lungs, pancreatic and prostate cancers. Elevated expression of NGFβ contributes to progression of human cholangiocarcinoma. In animal models, NGFβ is observed to prevent cholinergic neuron loss and enhances memory. NGF-β promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro. |
| General description: | The nerve growth factor β (NGFB) gene is mapped to human chromosome 1p13.2. |
| Physical form: | Lyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |