Denaturation Solution
SIGMA/N1531 - for Northern and Southern blotting
Synonym: Denaturation Solution For Blotting Technique; Denaturation Solution For Blotting Technique; Denaturation Solution for Neutral Southern Transfer; Denaturation Solution for Neutral Southern Transfer
Product Type: Chemical
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| form | solution |
| grade | Molecular Biology |
| Quality Level | 200  |
| storage temp. | room temp |
| Application: | Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures. |
| General description: | Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers. |
| Other Notes: | 0.5 N NaOH, 1.5 M NaCl |
| Other Notes: | The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl. |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H290 - H314 |
| Precautionary statements | P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 |
| Hazard Codes | C |
| Risk Statements | 34 |
| Safety Statements | 26-36/37/39-45 |
| RIDADR | UN 1824 8 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |