Anti-Nicastrin antibody produced in rabbit
SIGMA/N1660 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-ATAG1874
MDL Number: MFCD03791914
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Nicastrin antibody produced in Rabbit (Cat. No. N1660) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Nicastrin (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human Alzheimer′s Disease Brain sections with 1:100 Rabbit Anti-Nicastrin (Cat. No. N1660) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat brain sections with 1:100 Rabbit Anti-Nicastrin (Cat. No. N1660) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Whole extract of HEK293 cells was separated on SDS-PAGE and probed with 1:10,000 Rabbit Anti-Nicastrin antibody (Cat. No. N1660). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. The picture is courtesy of Matthias Voss, Regina Fluhrer and Prof. Christian Haass, Adolf-Butenandt Institute, LMU Munich & German Center for Neurodegenerative Diseases (DZNE), Munich, Germany.
Immunoblotting Lysates were separated on SDS-PAGE and probed with 1:1,000 Rabbit Anti-Nicastrin (Cat. No. N1660). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HEK-293 cells stably transfected with human Nicastrin 2. Negative control: non-transfected HEK-293 cells 3. Rat Brain
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 110 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:1,000 using minimum working antibody dilution using a whole cell extract of the HEK293 cell line stably transfected with human nicastrin. | |
| UniProt accession no. | Q92542  |
| Application: | Anti-Nicastrin antibody produced in rabbit has been used in: • western blotting • immunoprecipitation • co-immunoprecipitation |
| Biochem/physiol Actions: | Nicastrin has a key role in the regulation of presenilin-mediated cleavage of the b-amyloid precursor protein (b-APP) and Notch/GLP-1. Nicastrin binds to the membrane-tethered form of Notch and is essential for the intramembrane cleavage of Notch to generate Notch intracellular domain (NICD), which is involved in intracellular signaling. It has been suggested that nicastrin binds substrates of presenilin/g-secretase complexes or modulates g-secretase activity. Suppression of nicastrin expression in C. elegansembryos induces a subset of notch/glp-1 phenotypes similar to those induced by simultaneous null mutations in both presenilin homologues of C. elegans (sel-12 and hop-1). Thus, increasing evidence indicates that both nicastrin and presenilins are necessary components for the intramembrane proteolysis of proteins such as b-APP and Notch, and implicates a direct role for nicastrin in the pathogenesis of Alzheimer′s disease and in the regulation of Notch signaling in vivo. |
| Biochem/physiol Actions: | Rabbit polyclonal anti-Nicastrin recognizes human nicastrin (110 kDa) by immunoblotting. Staining of nicastrin in immunoblotting is specifically inhibited with nicastrin immunizing peptide. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Nicastrin (NCSTN) is a type I transmembrane glycoprotein (709 amino acids) that interacts with both presenilin-1 (PS1) and presenilin-2 (PS2). This gene is located on human chromosome 1q23. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of human nicastrin (amino acids 693-709) conjugated to KLH. The corresponding sequence is identical in mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |