5′-Nucleotidase human
SIGMA/N1665 - recombinant, expressed in CHO cells, vial of 6-12 μg
Synonym: CD73; NT5E; ecto-5′-nucleotidase
CAS Number: 9027-73-0
MDL Number: MFCD00130311
Product Type: Chemical
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| assay | ≥90% (SDS-PAGE) |
| description | contains a C-terminal 6-His tag |
| enzyme activity | ≥15 units/mg protein |
| form | solution |
| impurities | ≤1.0 EU/μg endotoxin |
| mol wt | ~61 kDa by SDS-PAGE (reducing) |
| NCBI accession no. | NP_002517   |
| packaging | vial of 6-12 μg |
| Quality Level | 200 |
| recombinant | expressed in CHO cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| Biochem/physiol Actions: | 5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophospha |
| Biochem/physiol Actions: | 5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis. |
| General description: | 5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH 9.0 at 37 °C. |
| Physical form: | Supplied as a solution containing Tris, NaCl, CaCl2, and 20% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | enzyme activity: ≥15 units/mg protein |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 3.1.3.5 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |