SIGMAFAST™ p-Nitrophenyl phosphate Tablets
SIGMA/N1891 - tablet, To prepare 5 mL
Synonym: pNPP tablets
MDL Number: MFCD00284586
Product Type: Chemical
pNPP substrates develop the yellow end-product, p-nitrophenol, when hydrolyzed by alkaline phosphatase. Since this system forms a soluble end product, it is not recommended for blotting or histochemistry.
| form | tablet |
| Quality Level | 200  |
| solubility | deionized water: soluble 5 mL |
| storage temp. | −20°C |
| Application: | Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets. |
| Application: | SIGMA FAST p-Nitrophenyl phosphate Tablets has been used: • to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2) • to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assay • as a substrate for ExtrAvidin® alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA) • as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC |
| Preparation Note: | Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |