Anti-NY-ESO-1 antibody, Mouse monoclonal
SIGMA/N2038 - clone E978, purified from hybridoma cell culture
Synonym: Anti-CTAG1; Anti-Cancer/Testis Antigen 1
MDL Number: MFCD09265426
Product Type: Chemical
Immunoblotting
Total cell extract of SK-Mel 37 cells were separated on SDS-PAGE and probed with Monoclonal Anti-NY-ESO-1 (Cat. No. N2038). The antibody was developed with Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. 16 μg/mL antibody
2. 2 μg/mL antibody
3. Negative control
Total cell extract of SK-Mel 37 cells were separated on SDS-PAGE and probed with Monoclonal Anti-NY-ESO-1 (Cat. No. N2038). The antibody was developed with Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. 16 μg/mL antibody
2. 2 μg/mL antibody
3. Negative control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | E978, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~23 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: 1-2 μg/mL using SKMel-37 total cell extract |
| Application: | Monoclonal Anti-NY-ESO-1 antibody produced in mouse has been used in enzyme linked immunosorbent assays (ELISA), immunoblotting and immunohistochemistry. |
| Application: | Monoclonal Anti-NY-ESO-1 is used as a probe to determine the presence and roles of Cancer/Testis Antigen 1 in humoral immune responses and a variety of cancers. |
| Biochem/physiol Actions: | Cancer/testis (CT) antigens are the protein products of genes that are activated in a wide variety of tumors and can elicit autologous cellular and humoral immune responses. Their normal expression is restricted to male germ cells in the testis and they are not expressed in adult somatic tissues. Therefore, CT antigens are promising candidates for cancer immunotherapy. Approximately 20 CT antigens or antigen families have been identified to date. NY-ESO-1 is a CT protein to which cell-mediated immune response has been demonstrated. It is highly expressed in various types of cancers. For example, patients with melanoma contain tumor-infiltrating lymphocytes cells (TILs) that recognized NY-ESO-1 protein. The expression of genes encoding CT antigens was examined in sporadic medullary thyroid carcinoma. The expression of NY-ESO-1 was found in 65 percent of the samples and correlated with tumor recurrence in primary MTC tumors. Furthermore, high frequency of expression was found in breast cancer especially in benign lesions and in multiple myeloma. Due to its wide expression in different cancers, NY-ESO-1 protein was used as a vaccine in cancer patients. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-NY-ESO-1 (mouse IgG1 isotype) is derived from the hybridoma E978 produced by the fusion of mouse myeloma cells (SP2/0) and splenocytes from BALB/c mice immunized with human recombinant NY-ESO-1. The NY-ESO-1, also known as cancer/testis antigen 1 is expressed in normal testis and to a much lower extent in placenta, ovary and uterus. |
| Immunogen: | recombinant human NY-ESO-1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |