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Naphthol AS-BI phosphate disodium salt hydrate

SIGMA/N2250 - ≥96.0% (HPLC)

Synonym: 2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-, disodium salt hydrate

CAS Number: 207569-04-8
Empirical Formula (Hill Notation): C18H13BrNNa2O6P · xH2O
Molecular Weight: 496.16 (anhydrous basis)
Linear Formula: C18H13BrNNa2O6P · xH2O
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-N2250-50MG 50 mg
$82.20
1/EA
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45-N2250-100MG 100 mg
$80.10
1/EA
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45-N2250-500MG 500 mg
$234.00
1/EA
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45-N2250-1G 1 g
$397.00
1/EA
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45-N2250-5G 5 g
$1500.00
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assay ≥96.0% (HPLC)
form powder
InChI 1S/C18H15BrNO6P.2Na.H2O/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24;;;/h2-10H,1H3,(H,20,21)(H2,22,23,24);;;1H2/q;2*+1;/p-2
InChI key WNPKMQCBZKBNOS-UHFFFAOYSA-L
Quality Level 200 
SMILES string O.[Na+].[Na+].COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP([O-])([O-])=O
solubility water: 50 mg/mL, clear, colorless to yellow
storage temp. −20°C
Application: Naphthol AS-BI phosphate disodium salt hydrate is suitable to use:
• in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
• as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
• to perform alkaline phosphatase reaction

Biochem/physiol Actions: Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.
General description: Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol.
General description: Substrate for the histochemical demonstration of acid and alkaline phosphatase.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥96.0% (HPLC)
Storage Temp. −20°C
UNSPSC 12352204

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