Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse
SIGMA/N2280 - clone NOS-B1, ascites fluid
Synonym: Anti-bNOS
MDL Number: MFCD01092550
Product Type: Chemical
Immunoblotting Rat cerebellum extract was separated on SDS-PAGE and probed with Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) (bNOS) Clone: NOS-B1 (Cat. No. N2280). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1:3,000 antibody 2. 1:6,000 antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NOS-B1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 150-160 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, goat, pig, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:3,000 using fresh rat cerebellum extract | |
| UniProt accession no. | P29475  |
| Application: | Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody produced in mouse has been used in: • western blot analysis • immunocytochemistry • double immunofluorescence • immunofluorescence staining • enzyme-linked immunosorbent assay (ELISA) • dot blot immunoassay • immunohistochemical staining |
| Biochem/physiol Actions: | Monoclonal Anti-Nitric Oxide Synthase, Brain (1-181) antibody is specific for nitric oxide synthase (NOS) derived from brain (bNOS, 150-160 kDa and several breakdown products of lower M.W.). The product does not bind to NOS derived from macrophages (mNOS) and endothelial cells (eNOS). This antibody is specific for bNOS in humans, goats, pigs and rats. |
| Biochem/physiol Actions: | Nitric oxide synthase is an enzyme that catalyzes the formation of nitric oxide. Nitric oxide is responsible for regulating several biochemical functions such as hemostasis, neurotransmission and vascular injury response . Mutations in nitric oxide synthase gene have been associated with diabetic nephropathy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Nitric Oxide Synthase-Brain (bNOS) (mouse IgG1 isotype) is derived from the NOS-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Nitric oxide synthase (NOS) has been localized in many different cell types. Type I NOS is found in neurons. It is a 150-160 kDa protein, also called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+- regulated NOS (cNOS). bNOS is present also in skeletal muscle, where it is complexed with dystrophin, and is absent in Duchenne′s muscular dystrophy, which perhaps accounts for symptoms of the disease. |
| Immunogen: | recombinant neuronal NOS fragment (amino acids 1-181) from rat brain. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |