Nucleoside Phosphorylase bacterial
SIGMA/N2415 - recombinant, expressed in E. coli, ≥10 units/mg protein
Synonym: PNP; Purine nucleoside phosphorylase; Purine nucleoside:orthophosphate ribosyltransferase
CAS Number: 9030-21-1
EC Number: 232-857-3
MDL Number: MFCD00131739
Product Type: Chemical
Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations. Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N2415-100UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N2415-1KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N2415-250UN
| assay | ≥80% protein basis (biuret) |
| form | lyophilized powder |
| InChI | 1S/C10H12N4O4/c15-2-6-7(1 |
| InChI key | MRWXACSTFXYYMV-FDDDBJFASA |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| sequence note | MATPHINAEMGDFADVVLMPGDPLR |
| SMILES string | [n]2(c3ncncc3nc2)[C@@H]1O |
| specific activity | ≥10 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P0ABP8  |
| Application: | Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-subst |
| Application: | Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate. |
| Application: | Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation. |
| Biochem/physiol Actions: | Catalyzes the following reaction: purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate |
| Biochem/physiol Actions: | Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations. |
| Other Notes: | One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C. |
| Packaging: | 100, 250, 1000 units in poly bottle |
| Preparation Note: | This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥80% protein basis (biuret) |
| activity | specific activity: ≥10 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.4.2.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |