NGF-β from rat
SIGMA/N2513 - recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture
Synonym: NGF-β from rat; Nerve Growth Factor-β from rat
CAS Number: 86923-98-0
MDL Number: MFCD00164982
Product Type: Chemical
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| assay | ≥97% (SDS-PAGE) |
| biological source | rat |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| packaging | pkg of 0.1 mg |
| potency | 0.15-3.00 ng/mL EC50 |
| Quality Level | 200  |
| recombinant | expressed in Sf21 cells |
| solubility | water: soluble 0.1 g/L at 20 °C |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P25427  |
| Analysis Note: | The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1. |
| Application: | NGF-β from rat is used for the following applications: • In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells) • Western blotting analysis • For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components) • In cell culture and treatment (for neuronal differentiation) • In the preparation of stock solution |
| Biochem/physiol Actions: | NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same. It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment. |
| General description: | NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells. It is a neurotrophin and belongs to the NGF family. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |