Nucleoside 5′-Diphosphate Kinase from bovine liver
SIGMA/N2635 - buffered aqueous glycerol solution, ≥1,000 units/mg protein (biuret)
Synonym: NDK; UDPkinase; nonmetastatic23(NM23); uridine diphosphate kinase; ATP:nucleoside diphosphate phosphotransferase; NDPK
CAS Number: 9026-51-1
MDL Number: MFCD00131734
Product Type: Chemical
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| biological source | bovine liver |
| foreign activity | lactic dehydrogenase, myokinase, β-NADH oxidase, nucleoside monophosphokinase and ATPase ≤0.1% |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| specific activity | ≥1,000 units/mg protein (biuret) |
| storage condition | (Tightly closed) |
| storage temp. | 2-8°C |
| technique(s) | activity assay: suitable |
| Application: | Nucleoside 5′-Diphosphate Kinase has been used:• nucleoside diphosphate kinase (NDPK) assays • coupled NDPK-luciferase assay to determine the amounts of Ras-bound guanosine triphosphate (GTP) • non-metastatic protein 23 (NM23) growth stimulation assay • in a study to assess inhibition of type I Fc epsilon receptor mediated Ca2+ influx and mediator secretion in rat mucosal mast cells • in a study to investigate protein synthesis in rabbit reticulocytes |
| Biochem/physiol Actions: | Nucleoside 5′-Diphosphate Kinases (NDKs) aid in the synthesis of nucleoside triphosphates (NTPs) by transferring a phosphate group from ATP to nucleoside diphosphates (NDPs). NDKs provide NTPs for nucleic acid synthesis, cytidine 5′-triphosphate (CTP) for lipid synthesis, uridine 5′-triphosphate (UTP) for polysaccharide synthesis, and guanosine triphosphate (GTP) for protein elongation, signal transduction, and microtubule polymerization. |
| General description: | Research area: Cell Signaling Nucleoside 5′-Diphosphate Kinase (NDK) is a ubiquitous housekeeping enzyme. Nucleoside Diphosphate Kinase exists in two isoforms in eukaryotic cells, NDK-A and NDK-B. These enzymes are found expressed both in the mitochondria and the cytoplasm. |
| Other Notes: | One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH. |
| Packaging: | 100, 500 units in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1,000 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.7.4.6 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |