4-Nitrophenyl phosphate disodium salt hexahydrate
SIGMA/N2765 - tablet
Synonym: p-Nitrophenyl phosphate disodium hexahydrate; Disodium 4-nitrophenyl phosphate hexahydrate; di-Sodium 4-nitrophenyl phosphate; pNPP disodium hexahydrate
CAS Number: 333338-18-4
Empirical Formula (Hill Notation): C6H4NNa2O6P · 6H2O
Molecular Weight: 371.14
EC Number: 224-246-5
MDL Number: MFCD00066288
Linear Formula: O2NC6H4OP(O)(ONa)2 · 6H2O
Product Type: Chemical
| form | tablet |
| InChI | 1S/C6H6NO6P.2Na.6H2O/c8-7 |
| InChI key | KAKKHKRHCKCAGH-UHFFFAOYSA |
| Quality Level | 300 ![]() |
| SMILES string | O.O.O.O.O.O.[Na+].[Na+].[ |
| solubility | water, high purity: 0.25 tablet/mL, clear, colorless |
| storage temp. | −20°C |
| Application: | 4-Nitrophenyl phosphate disodium salt hexahydrate has been used: • as a substrate for alkaline phosphatase coupled anti-rabbit antibody in enzyme-linked immunosorbent assay (ELISA) • as a substrate for the mouse secondary antibody linked to alkaline phosphatase in immunoenzymometric assay • in ELISA to analyze the presence of antibodies to Pseudomonas aeruginosa in animal serum sample |
| General description: | Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm. |
| Preparation Note: | Contains 20 mg substrate per tablet |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |

