Monoclonal Anti-Neurofilament 160 antibody produced in mouse
SIGMA/N2787 - ~2 mg/mL, clone RMO44, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Neurofilament Medium Chain
MDL Number: MFCD01322393
Product Type: Chemical
Western Blotting Whole extract of SHSy5y Human neuroblastoma cells were separated on SDS-PAGE and probed with Monoclonal Anti-Neurofilament 160 antibody produced in Mouse, Clone: RMO44 (Cat. No. N2787). The antibody was developed using 1:2,500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 2 μg/mL antibody 2. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | RMO44, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat, zebrafish |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| microarray: suitable | |
| western blot: 1-2 μg/mL using extract of SHSy5y human neuroblastoma cells | |
| UniProt accession no. | P07197  |
| Application: | Monoclonal Anti-Neurofilament 160 antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • western blot analysis • immunohistochemistry • antibody labelling |
| Biochem/physiol Actions: | Neurofilament 160 (NEFM) associates with the dopamine receptor. |
| Biochem/physiol Actions: | Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modifications, which results in their heterogeneity including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing their interaction with cytoplasmic organelles. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti- Neurofilament 160 (mouse IgG1 isotype) is derived from the hybridoma RMO44 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified mid-sized rat neurofilament (NF-M) subunit. Neurofilaments are one of the five major groups of intermediate filaments (Ifs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kDa, 160 kDa and 200 kDa. |
| General description: | Neurofilament 160 (NEFM) is one among the most abundant filaments in post-mitotic neurons. |
| Immunogen: | purified mid-sized rat neurofilament (NF-M) subunit. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |