Neuraminidase from Clostridium perfringens (C. welchii)
SIGMA/N2876 - Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder
Synonym: Acyl-neuraminyl Hydrolase; Receptor-destroying enzyme; Sialidase
CAS Number: 9001-67-6
EC Number: 232-624-6
MDL Number: MFCD00131711
Product Type: Chemical
Neuraminidase non-specific cleavage for sialic acid — α(2-3), α(2-6) and α(2-8) glycosiduic linkages with a variety of natural and synthetic substrates
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N2876-80320UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N2876-250UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N2876-25UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N2876-625UN
| application(s) | diagnostic assay manufacturing |
| foreign activity | Protease and NAN-aldolase, present |
| form | lyophilized powder |
| Quality Level | 300  |
| shipped in | dry ice |
| specific activity | ≥0.1 units/mg solid (using mucin) |
| ≥1.3 units/mg solid (using 4MU-NANA) | |
| storage temp. | −20°C |
| type | Type V |
| Analysis Note: | Package sizes based on 4MU-NANA units |
| Analysis Note: | Package sizes based on the 4MU-NANA units |
| Application: | Neuraminidase from Clostridium perfringens has been used in a study to assess binding with human T lymphocytes in sheep pretreated with neuraminidase. It has also been used in a study to investigate the effect of bile salts on the action of hydrolysis by neuraminidase. |
| Biochem/physiol Actions: | Neuraminidase can increase aggregation in certain cell lines by removing exposed negatively charged sialic acid residues on the cell surface. |
| Biochem/physiol Actions: | Neuraminidase cleavage of sialic acid groups has been used to study recognition by antibodies of glycoprotein structures. The use of neuraminidase in the estimation of N-acetylneuraminic acid was compared favorably to two other methods. |
| Biochem/physiol Actions: | Neuraminidases are used to cleave terminal N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins. The enzyme from Clostridium perfringens cleaves terminal sialic acid residues which are α-2,3- α-2,6- or α-2,8-linked to Gal, GlcNac, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids or glycoproteins. The relative rate of cleavage decreases in the order: α-2-3 > α-2-6 . α-2-8. Neuraminidase from C. perfringens cleaves α-2-3 linked sialic acid residues most efficiently, compared to A. ureafaciens, (Sigma N3642) which preferentially cleaves α-2-6 linked residues. |
| Biochem/physiol Actions: | The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37°C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 °C. |
| General description: | Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread. |
| Other Notes: | One unit will liberate 1.0 micromole of N-acetyl neuraminic acid per minute at pH 5.0 at 37 °C using bovine submaxillary mucin. One unit will hydrolyze 1.0 micromole of 2′-(4-methylumbelliferyl) |
| Packaging: | 2.5, 6 units in glass bottle |
| Packaging: | 25, 250 units in poly bottle |
| Preparation Note: | Prepared by salt fractionation. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.1 units/mg solid (using mucin); specific activity: ≥1.3 units/mg solid (using 4MU-NANA) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.18 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |