Anti-Neurofilament 160/200 antibody, Mouse monoclonal
SIGMA/N2912 - ~2 mg/mL, clone RMdO20, purified from hybridoma cell culture
Synonym: Anti-NEF3
MDL Number: MFCD00164708
Product Type: Chemical
Immunofluorescence A transverse section of a Rat lumbar dorsal root ganglion stained with fluorescent markers to distinguish subpopulations of sensory neurons with distinct roles in the pain pathway. Large myelinated neurons and associated fibers are immunoreactive for neurofilament-200 (NF200, in green), while small neurons are divided into peptidergic (in red) and non-peptidercgic (in blue) nociceptors expressing calcitonin gene-related peptide (CGRP) or isolectin B4 (IB4), respectively. Small neurons can encode the intensity of a painful stimulus into firing frequencies, while large neurons are thought to principally mediate sensations of touch, pressure and proprioception. Submitted by Dr C. Tsantoulas, University of Cambridge
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-Neurofilament 160/200 Clone: RMdO20 (Cat. No. N2912). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. SH-SY5Y 4. SK-N-SH 5. COS7
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | RMdO20, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using extracts of rat brain S1 fraction. | |
| UniProt accession no. | P07197  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Application: | Monoclonal anti-neurofilament 160/200 antibody can be used in immunocytochemistry (diluted 1: 100) and western blotting (diluted 1: 1,000) . It can also be used in immunohistochemistry. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Neurofilaments are 10 nm in diameter and belong to intermediate filament protein family. Neurofilaments are expressed mainly in cells or tissues of neuronal origin and are crucial for proper radial growth of axons. Monoclonal anti-neurofilament 160/200 antibody can be used in ELISA and immunoblotting. It can also be used in microarray. Mouse anti-neurofilament 160/200 antibody reacts specifically with the non-phosphorylated form of NF-M and NF-H (NF-M/H, approx.160 and 200 kDa) in mouse, rat and human. |
| Immunogen: | purified mid-size rat neurofilament (NF-M) subunit. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |