Neuraminidase from Clostridium perfringens (C. welchii)
SIGMA/N3001 - Type VI, lyophilized powder, 6-15 units/mg protein (using 4MU-NANA), 2-10 units/mg protein (mucin)
Synonym: Acyl-neuraminyl Hydrolase; Receptor-destroying enzyme; Sialidase
CAS Number: 9001-67-6
EC Number: 232-624-6
MDL Number: MFCD00131711
Product Type: Chemical
Neuraminidase non-specific cleavage for sialic acid — α(2-3), α(2-6) and α(2-8) glycosiduic linkages with a variety of natural and synthetic substrates
| biological source | Clostridium perfringens str. 13 |
| composition | Protein, ≥50% biuret |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | 2-10 units/mg protein (mucin) |
| 6-15 units/mg protein (using 4MU-NANA) | |
| storage temp. | −20°C |
| type | Type VI |
| Analysis Note: | Package sizes based on 4MU-NANA units |
| Analysis Note: | Package sizes based on the 4MU-NANA units |
| Application: | Neuraminidase from Clostridium perfringens (C. welchii) has been used in a study to assess a glycoprotein faction suitable for use as a substrate in preparation assays. It has also been used in a study to investigate the action of an epsilion-toxin on MDCK cells. |
| Biochem/physiol Actions: | Neuraminidase cleavage of sialic acid groups has been used to study recognition by antibodies of glycoprotein structures. The use of neuraminidase in the estimation of N-acetylneuraminic acid was compared favorably to two other methods. |
| Biochem/physiol Actions: | Neuraminidase from Clostridium perfringens reduces the viability of human leukemic myeloblasts and attenuates their ability to activate lymphocytes. |
| Biochem/physiol Actions: | Neuraminidases are used to cleave terminal N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins. The enzyme from Clostridium perfringens cleaves terminal sialic acid residues which are α-2,3- α-2,6- or α-2,8-linked to Gal, GlcNac, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids or glycoproteins. The relative rate of cleavage decreases in the order: α-2-3 > α-2-6 . α-2-8. Neuraminidase from C. perfringens cleaves α-2-3 linked sialic acid residues most efficiently, compared to A. ureafaciens, (Sigma N3642) which preferentially cleaves α-2-6 linked residues. |
| Biochem/physiol Actions: | The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37°C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 °C. |
| General description: | Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread. |
| Packaging: | 4, 10 units in glass bottle |
| Preparation Note: | Chromatographically purified from Type V (N 2876) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 2-10 units/mg protein (mucin); specific activity: 6-15 units/mg protein (using 4MU-NANA) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.18 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |