Anti-Nanog antibody, Mouse monoclonal
SIGMA/N3038 - clone NNG-811, purified from hybridoma cell culture
Synonym: Anti-NANOG/STM1
Product Type: Chemical
Immunofluorescence 3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with Monoclonal Anti-Nanog (5 mg/mL) (Cat. No. N3038) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.
Immunoblotting Total cell extract of NT2 cells (20 μg/ lane) was separated on SDS-PAGE and blotted with Monoclonal Anti-Nanog (Cat. No. N3038). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1: 10 μg/mL antibody 2: 2 μg/mL antibody 3: Negative control – no primary antibody
Immunoprecipitation Monoclonal Anti-Nanog Clone: NNG-811 (Cat. No. N3038) was used to immunoprecipitate Nanog from NT2 whole cell extract. Detection antibody: Monoclonal Anti-Nanog Clone: NNG-811 (Cat. No. N3038). Lanes 1. 5 μg antibody 2. 10 μg antibody 3. Negative control: without cell extract 4. Positive control: NT2 whole cell extract
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NNG-811, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | ~40 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 4-8 μg/mL | |
| UniProt accession no. | Q9H9S0  |
| Application: | Anti-Nanog antibody, Mouse monoclonal has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • immunocytochemistry • flow cytometric analysis • immunoprecipitation • immunofluorescence |
| Application: | Monoclonal Anti-Nanog antibody is suitable for use in ELISA, immunoblotting (~ 40 kDa), immunocytochemistry and immunoprecipitation. Immunoblotting: a working antibody concentration of 2-4 mg/mL is recommended using extracts of NT2 cells. |
| Biochem/physiol Actions: | Monoclonal Anti-Nanog reacts specifically with human Nanog. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Nanog antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma NNG-811 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human Nanog. The human Nanog gene product is a 305 amino acid, 35 kDa protein with a tryptophan repeat domain and two C-terminal trans-activating domains. It is primarily located in the nucleus. |
| General description: | Nanog is a homeodomain transcription factor expressed in cells of the inner cell mass of early embryos, in embryonic stem cells (ESC), germ cells and in stem/progenitor cells of some tissues. Together with Oct-4, Sox-2, and Rex-1, it is a molecular marker for pluripotent cells and for undifferentiated stem cells. This protein is crucial for the maintenance of pluripotency of ESCs and is down-regulated when ESCs differentiate. Nanog controls the expression of many ESC genes together with other stem cell transcription factors like Oct-4 and Sox-2. Nanog targets both repressor and activator complexes to regulatory regions of hundreds of genes in the genome. Expression of nanog can be detected primarily in germ cell tumors and in tumors of other cell types. Nanog is an important marker for Seminomas, testicular carcinomas, teratocarcinomas, and germ cell-like tumors in various tissues. Furthermore, it was shown to transform NIH3T3 cells. |
| Immunogen: | recombinant human Nanog. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |