4-Nitrophenyl phosphate bis(cyclohexylammonium) salt
SIGMA/N3129 - phosphatase substrate
Synonym: p-Nitrophenyl phosphate bis(cyclohexylammonium) salt; PNPP
CAS Number: 52483-84-8
Empirical Formula (Hill Notation): C6H6NO6P · 2C6H13N
Molecular Weight: 417.44
EC Number: 257-949-0
MDL Number: MFCD00013173
Linear Formula: O2NC6H4OP(O)(O-)2 · 2C6H11NH3+
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N3129-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N3129-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N3129-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N3129-5G
| assay | ≥97% anhydrous basis |
| form | powder |
| InChI | 1S/C6H6NO6P.2C6H13N/c8-7( |
| InChI key | KXNNUYCXODNFBD-UHFFFAOYSA |
| Quality Level | 300  |
| SMILES string | NC1CCCCC1.NC2CCCCC2.OP(O) |
| solubility | water: 10 mg/mL, clear, colorless to faintly yellow |
| storage temp. | −20°C |
| Application: | 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay. |
| Biochem/physiol Actions: | p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm. |
| Packaging: | 1, 5, 25, 100 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% anhydrous basis |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |