Anti-NBS1 (Nibrin) antibody produced in rabbit
SIGMA/N3162 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-p95 Protein of the MRE11/RAD50 Complex
MDL Number: MFCD07784851
Product Type: Chemical
Immunoblotting
Extracts of HeLa nucleus (Lane 1), Hela cells (Lane 2), Mcf7 nucleus (Lane 3), Mcf7 cells (Lane 4), PC12 cells (Lane 5), and C2 cells (Lane 6) were separated on SDS-PAGE and blotted with Anti-NBS1 (Nibrin) (Cat. No. N3162). The antibody was developed with with Anti-Rabbit IgG, Peroxidase and a chemiluminescent substrate.
Extracts of HeLa nucleus (Lane 1), Hela cells (Lane 2), Mcf7 nucleus (Lane 3), Mcf7 cells (Lane 4), PC12 cells (Lane 5), and C2 cells (Lane 6) were separated on SDS-PAGE and blotted with Anti-NBS1 (Nibrin) (Cat. No. N3162). The antibody was developed with with Anti-Rabbit IgG, Peroxidase and a chemiluminescent substrate.
Immunofluorescence Nuclear staining of NBS1 in HEK-293T cells transfected with NBS1 expression plasmid. Cells were stained with Rabbit Anti-NBS1 (Nibrin) (Cat. No. N3162).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 95 kDa (also recognizes 100 kDa band) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | canine, human, rat, chimpanzee |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg |
| indirect immunofluorescence: 5-10 μg/mL using Hela cells, fixed with paraformaldehyde/triton | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using MCF7 nuclear extracts | |
| UniProt accession no. | O60934  |
| Application: | Anti-NBS1 (Nibrin) antibody has been used: • in immunofluorence • in immunoblotting • in immunoprecipitation |
| Biochem/physiol Actions: | NBS1 (Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with Nijmegen breakage syndrome (NBS). The majority of NBS patients bear a five base pairs deletion that leads to truncated NBS1, called 657del5. p95/NBS1 (nibrin) deficiency abrogates the formation of the MRE11/RAD50 ionizing radiation-induced foci, revealing a molecular link between DSB repair and cell cycle checkpoints activated by DNA damage. |
| Biochem/physiol Actions: | Nibrin (NBS1) is involved in stabilizing genomes and has a role in development of cancers. It also takes part in the repair mechanism after double strand breaks in the DNA. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NBS1 (Nibrin), also known as p95 protein of the meiotic recombination 11 homolog 1/DNA repair protein RAD50 (MRE11/RAD50) complex. NBS1 contains two domains found in the cell cycle checkpoint proteins forkhead-associated domain (FHA) and an adjacent breast cancer carboxyterminal domain (BRCT). NBS1 is one of the protein components of the double-strand break repair complex NBS1/MRE11/p50. |
| General description: | Nibrin (NBS1) is made up of 754 amino acids. |
| Immunogen: | synthetic peptide corresponding to amino acids 692-706 of mouse NBS1 (nibrin), conjugated to KLH via an N-terminal added cysteine residue. |
| Physical form: | Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |