Advanced Search



4-Nitrophenyl phosphate di(tris) salt

SIGMA/N3254 - phosphatase substrate

Synonym: 4-Nitrophenyl phosphate bis(tris) salt

CAS Number: 68189-42-4
Empirical Formula (Hill Notation): C14H28N3O12P
Molecular Weight: 461.36
EC Number: 269-198-6
MDL Number: MFCD00037009
Linear Formula: (C6 H4 N O6 P)( C4 H12 N O3)2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-N3254-1G 1 g
 $62.30
1/EA		 Add To Favorites
45-N3254-5G 5 g
 $106.00
1/EA		 Add To Favorites
45-N3254-25G 25 g
 $424.00
1/EA		 Add To Favorites
45-N3254-100G 100 g
 $1090.00
1/EA		 Add To Favorites

 

assay ≥97% (enzymatic)
form powder
InChI 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key XXAXKCWOTRABOW-UHFFFAOYSA-N
Quality Level 100 
SMILES string NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
solubility water: 50 mg/mL, clear, colorless to light yellow
storage temp. −20°C
Application: 4-Nitrophenyl phosphate di(tris) salt has been used:
• as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
• to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
• for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions: p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥97% (enzymatic)
Storage Temp. −20°C
UNSPSC 12352204

The following items have been added to your cart:

Choose a favorite list for this item:
Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top