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Naphthol Blue Black

SIGMA/N3393 - BioReagent, suitable for electrophoresis

Synonym: Acid Black 1; Amido Black 10B; Buffalo Black NBR

CAS Number: 1064-48-8
Empirical Formula (Hill Notation): C22H14N6Na2O9S2
Molecular Weight: 616.49
EC Number: 213-903-1
MDL Number: MFCD00004017
Linear Formula: C22H14N6Na2O9S2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-N3393-25G 25 g
 $60.50
1/EA		 Add To Favorites
45-N3393-100G 100 g
 $164.00
1/EA		 Add To Favorites
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N3393-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N3393-25G

 

composition Dye content, ~85%
form powder
impurities ≤10% water
InChI 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
InChI key AOMZHDJXSYHPKS-DROYEMJCSA-L
product line BioReagent
Quality Level 200 
SMILES string [Na+].[Na+].Nc1c(N=Nc2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(N=Nc4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
suitability suitable for electrophoresis
technique(s) protein staining: suitable
Application: Naphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.
Biochem/physiol Actions: Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.
Packaging: 25, 100 g in glass bottle
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Colour Index Number 20470
UNSPSC 41105322

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