Nutrient Mixture F-12 Ham
SIGMA/N3520 - Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym: Ham’s F-12
MDL Number: MFCD00217417
Product Type: Chemical
| components | NaHCO3: no sodium pyruvate: 0.22 g/L HEPES: no L-glutamine: yes phenol red: yes |
| form | powder |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Nutrient Mixture F-12 Ham is used for the following applications: • HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum • Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components) • Used in media for the propagation of Cowdria ruminantium • Used during preparation of primary mixed glial cultures Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells. |
| General description: | The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells. |
| Preparation Note: | Formulated to contain 11.1 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 2.5 g/L sodium bicarbonate. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |