Nuclease micrococcal from Staphylococcus aureus
SIGMA/N3755 - 100-300 units/mg protein
Synonym: Micrococcal nuclease; Endonuclease micrococcal; MNase; Micrococcal endonuclease
CAS Number: 9013-53-0
EC Number: 232-748-0
MDL Number: MFCD00131731
Product Type: Chemical
Catalyzes the exo- and endonucleolytic cleavage of both double- and single-stranded DNA and RNA, with preference for AT and AU-rich regions, yielding nucleoside 3′-phosphates and 3′-phosphooligonucleotide end-products. It is most active against single-stranded DNA.
| application(s) | diagnostic assay manufacturing |
| biological source | Staphylococcus aureus |
| form | lyophilized powder |
| Quality Level | 300  |
| specific activity | 100-300 units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | DNA purification: suitable |
| Application: | Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases. |
| Biochem/physiol Actions: | Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C. |
| Biochem/physiol Actions: | Hydrolyzes 5′-phosphodiester bonds of DNA. |
| Other Notes: | Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm. |
| Other Notes: | One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on E |
| Packaging: | 50, 200, 500 units in serum bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 100-300 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.31.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |