α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens
SIGMA/N3786 - Proteomics Grade, suitable for MALDI-TOF MS
Synonym: Neuraminidase from Arthrobacter ureafaciens; Acyl-neuraminyl Hydrolase; Sialidase
CAS Number: 9001-67-6
EC Number: 232-624-6
MDL Number: MFCD00131711
Product Type: Chemical
Figure 1. Exoglycosidase enzymes commonly used to determine the structure of N-linked glycans by sequential degradation.
| form | lyophilized powder |
| quality | Proteomics Grade |
| Quality Level | 200  |
| specific activity | ≥25 U/vial |
| storage temp. | 2-8°C |
| suitability | suitable for MALDI-TOF MS |
| Analysis Note: | The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity. |
| Application: | Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein. |
| Other Notes: | One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α- |
| Packaging: | Provided with 5× concentrate reaction buffer. |
| Physical form: | Lyophilized powder |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥25 U/vial |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.18 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |
| Components | Neuraminidase 5X Reaction Buffer 1.5 mL |