Anti-Nitric Oxide Synthase, Endothelial (1185-1205) antibody produced in rabbit
SIGMA/N3893 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-eNOS
MDL Number: MFCD01092428
Product Type: Chemical
Immunohistochemistry Staining of acetone-fixed, frozen tissue sections of mouse heart with 1:100 Rabbit Anti-Nitric Oxide Synthase, Endothelial (1185-1205) (Cat. No. N3893) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Whole lysate of HeLa cells was separated on SDS-PAGE and probed with Rabbit Anti-Nitric Oxide Synthase, Endothelial (1185-1205) (Cat. No. N3893). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody dilution: 1:1,000
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 135 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): 1:100 using acetone-fixed, frozen tissue sections of mouse heart |
| microarray: suitable | |
| western blot: 1:10,000 using bovine lung endothelial cell extract | |
| UniProt accession no. | P29474  |
| Application: | Anti-Nitric Oxide Synthase, Endothelial (1185-1205) antibody produced in rabbit has been used in immunoblotting and immunohistochemistry. |
| Biochem/physiol Actions: | Endothelial nitric oxide synthase (eNOS) has been studied as a modulator of vascular function and it is involved in the production of nitric oxide. Defects in the enzyme expression have been shown to be associated with coronary artery disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Endothelial nitric oxide synthase (eNOS) is a 135 kDa protein and is an isoform of NOS. eNOS expression is not limited to the endothelium of blood vessels, it is also expressed in the epithelium of several tissues, including the bronchial tree. It is also localized in neurons in the brain, especially the pyramidal cells of the hippocampus. |
| General description: | The gene encoding endothelial nitric oxide synthase (eNOS) is localized on chromosome 7q35-36 and has 26 exons. |
| Immunogen: | synthetic peptide corresponding to nitric oxide synthase (NOS) of bovine endothelial origin (eNOS, amino acids 1185-1205 with an N-terminally added lysine) conjugated to KLH. The immunogen sequence is highly conserved in human eNOS. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |