Anti-NAV3 (N-terminal) antibody produced in rabbit
SIGMA/N4038 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Neuron navigator 3; Anti-Pore membrane and/or filament interacting like protein 1 (POMFIL1); Anti-STEERIN3; Anti-human UNC-53 homolog (unc53H3)
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-NAV3 (N-terminal) antibody produced in Rabbit (Cat. No. N4038) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing NAV3 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Western Blotting HEK-293T cells lysate expressing Human NAV3 fusion protein (lanes 1,3) and of HEK-293T non transfected (lane 2), were separated on SDS-PAGE, blotted with Anti-NAV3 (N-terminal) antibody produced in Rabbit (Cat. No. N4038) and developed using 1:10,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 1:1,000 2. Antibody: 1:1,000 3. Antibody: 1:1,000 + NAV3 immunizing peptide (human, 939-956)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~280 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat (predicted), human, mouse (predicted) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using HEK-293T cells lysate expressing human NAV3 fusion protein |
| UniProt accession no. | Q8IVL0  |
| Biochem/physiol Actions: | Anti-NAV3 (N-terminal) specifically recognizes human NAV3. |
| Biochem/physiol Actions: | NAV3 (neuron navigator 3) has been suggested to function as a novel tumor suppressor gene. It is a putative haploin sufficient tumor suppressor gene, which helps in the growth, differentiation and apoptosis of cutaneous T-cell lymphoma (CTCL)cells. |
| Biochem/physiol Actions: | NAV3 gene deletions and translocation affect patients with advanced mycosis fungoides or Sezary syndrome, the most common forms of primary cutaneous T-cell lymphoma (CTCL), suggesting that it may provide a novel diagnostic tool. |
| Biochem/physiol Actions: | Neuron navigator 3 (NAV3) has been shown to control migration and invasion of epithelial cells. Once it is activated by growth factors, NAV3 occupies the ends of microtubules and stimulates their growth. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NAV3 (neuron navigator 3, also known as unc-53H3, steerin3, POMFIL1) belongs to the neuron navigator (NAV) family of proteins. NAV proteins are predominantly expressed in the nervous system. NAV3 expression is detected in brain. |
| General description: | NAV3 is made up of a calponin homology (CH) domain, a cytoskeletal interacting domain (CSID), four coiled-coil (CC) domains, and an AAA-domain. |
| General description: | Neuron navigator 3 (NAV3) is highly expressed in tumors. It possesses actin-binding domains and the gene encoding it is localized on chromosome 12q21. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |