Anti-Neurofilament 200 antibody produced in rabbit
SIGMA/N4142 - IgG fraction of antiserum, buffered aqueous solution
Synonym: NF200 Antibody - Anti-Neurofilament 200 antibody produced in rabbit; Nf200 Antibody; AntiNeurofilament heavy chain
MDL Number: MFCD02263444
Product Type: Chemical
Immunofluorescence
Primary neuronal culture of rat amygdala. Nuclei stained blue with DAPI (Cat. No. D9542), neuron cluster (lower left) ensheathed by gail processes stained red with Rabbit Anti-GFAP (Cat. No. G9269) and Goat Anti-Rabbit IgG-TRITC (Cat. No. T6778) and positive neuronal fibers stained green with Rabbit Anti-Neurofilament 200 (Cat. No. N4142) and Goat Anti-Rabbit IgG-FITC (Cat. No. F9887).
From R. Gabr, West Virginia Univ., Dept of Pharmacology & Toxicology, Morgantown, WV.
Primary neuronal culture of rat amygdala. Nuclei stained blue with DAPI (Cat. No. D9542), neuron cluster (lower left) ensheathed by gail processes stained red with Rabbit Anti-GFAP (Cat. No. G9269) and Goat Anti-Rabbit IgG-TRITC (Cat. No. T6778) and positive neuronal fibers stained green with Rabbit Anti-Neurofilament 200 (Cat. No. N4142) and Goat Anti-Rabbit IgG-FITC (Cat. No. F9887).
From R. Gabr, West Virginia Univ., Dept of Pharmacology & Toxicology, Morgantown, WV.
Immunofluorescence
Fetal rat amygdala neurons in culture. Nuclei stained blue with DAPI (Cat. No. D9542), and neural filaments stained green with Rabbit Anti-Neurofilament 200 (Cat. No. N4142) and Goat Anti-IgG-FITC (Cat. No. F9887).
From R. Gabr, West Virginia Univ., Dept of Pharmacology & Toxicology, Morgantown, WV.
Fetal rat amygdala neurons in culture. Nuclei stained blue with DAPI (Cat. No. D9542), and neural filaments stained green with Rabbit Anti-Neurofilament 200 (Cat. No. N4142) and Goat Anti-IgG-FITC (Cat. No. F9887).
From R. Gabr, West Virginia Univ., Dept of Pharmacology & Toxicology, Morgantown, WV.
Enhanced Validation-By Independent Antibodies Formalin-fixed, paraffin-embedded Rat cerebellum tissue sections were stained with 1:80 Anti-Neurofilament 200 antibody produced in Rabbit (Cat. No. N4142) (A). The antibody was developed using 1:50 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887), and 1:40 Monoclonal Anti-Neurofilament 200 antibody produced in Mouse, Clone: NE14 (Cat. No. N5389) (B). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262). Results Two Anti-Neurofilament 200 antibodies, N4142 (A) and N5389 (B) target different regions of Neurofilament 200 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of rat cerebellum stained with 1:80 Anti-Neurofilament 200 antibody produced in rabbit (Cat. No. N4142) followed by Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat (Cat. No. F9887).
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent ( Antibodies) Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 200 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | bovine, wide range |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections |
| microarray: suitable | |
| western blot: suitable |
| Application: | Anti-neurofilament 200 antibody produced in rabbit has been used for immunolabeling. Anti-neurofilament 200 antibody produced in rabbit has been used: • for detection of neurofilaments using immunohistological staining • in human astrocytoma cell line • in post-mortem brain sections • in human cremaster muscles • for detection of neurofilaments by immunofluorescence staining in lumbar spinal cord sections of mice |
| Biochem/physiol Actions: | Neurofilament 200 plays a vital role in cytoarchitecture organization. Downregulated expression of the gene has been observed in schizophrenia patients. Loss of gene expression is associated with the development of prostate carcinoma. Mutation in the gene leads to sporadic amyotrophic lateral sclerosis (ALS). Neurofilament 200 assembly during development is critical for normal functioning of neuronal cells. Mutations in gene encoding neurofilaments is associated with neurological disorder called the Charcot–Marie–Tooth. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Neurofilament 200, also known as neurofilament heavy polypeptide (NEFH), is encoded by the gene mapped to human chromosome 22q12.2. The encoded protein has a molecular mass of 200kDa and belongs to the group of type IV intermediate filaments which are key constituents of the neuronal cytoskeleton. NEFH contains C-terminal motifs involved in the formation of sidearm projections which cross-link the neurofilaments. |
| Immunogen: | Neurofilament 200 from bovine spinal cord. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Shows a wide species cross-reactivity. Reacts with neurofilaments in the central and peripheral nervous system and localizes the neurofilament polypeptide. |
| Storage and Stability: | Store at -20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, solution may be frozen in working aliquots at –20°C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |