Nek2, Active human
SIGMA/N4787 - recombinant, expressed in E. coli, ≥85% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: Never In Mitosis Gene A-Related Kinase 2; Nima-Related Kinase 2
MDL Number: MFCD07370971
Product Type: Chemical
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| assay | ≥85% (SDS-PAGE) |
| enzyme activity | ≥10 units/mg protein |
| form | buffered aqueous glycerol solution |
| mol wt | protein apparent mol wt ~80 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | P51955  |
| Application: | Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP. |
| Biochem/physiol Actions: | Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome. |
| Other Notes: | One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP |
| Physical form: | Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥85% (SDS-PAGE) |
| activity | enzyme activity: ≥10 units/mg protein |
| Storage Temp. | −70°C |
| UNSPSC | 51111800 |