Monoclonal Anti-Neurofilament 68 antibody produced in mouse
SIGMA/N5139 - clone NR4, ascites fluid
Synonym: Monoclonal Anti-Neurofilament light chain
MDL Number: MFCD02097017
Product Type: Chemical
Immunohistochemistry Frozen sections acetone fixed Rat cerebellum tissue sections were stained with 1:400 Monoclonal Anti-Neurofilament 68 antibody produced in Mouse, Clone: NR4 (Cat. No. N5139), followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:400 Monoclonal Anti-Neurofilament 68, Clone: NR4 (Cat. No. N5139). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. PC3 2. SHSY-5Y 3. HeLa 4. MDBK 5. MDCK
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NR4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide as preservative |
| isotype | IgG1 |
| mol wt | antigen apparent mol wt 68 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, rat, pig, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using rat cerebellum |
| microarray: suitable | |
| western blot: 1:500 using bovine spinal cord neurofilament | |
| UniProt accession no. | P07196  |
| Application: | Monoclonal Anti-Neurofilament 68 antibody has been used: • in immunohistochemistry • in immunoblotting • in western blotting |
| Application: | Mouse Monoclonal clone NR4 anti-Neurofilament 68 antibody may be used for the localization of the neurofilament of molecular weight equal to 68,000 while being non-reactive with other intermediate filament proteins. |
| Biochem/physiol Actions: | Neurofilaments undergo post-translational modification, which results in their heterogeneity, including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles. Neurofilaments play a role in axonal calibre as they help in movement of an impulse down the axon. Their activity depends on phosphorylation of neurofilaments.Mutations of neurofilaments causes Charcot-Marie-Tooth (CMT) disease. Accumulation of neurofilaments has been observed in many human neurological diseases like Alzheimer′s disease, progressive supranuclear palsy, diabetic neuropathy, and giant axonal neuropathy. |
| Biochem/physiol Actions: | The antibody reacts specifically with neurofilament 68 in cultured cells or tissue preparations originating from human, pig, rat or chicken. It does not cross react with other intermediate filament proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monclonal Anti-Neurofilament 68 (mouse IgG1 isotype) is produced by the fusion mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are composed of three sub-units- light NFL protein, medium NFM protein, heavy NFH protein. Along with there two other intermediate filaments are present α-internexin and peripherin. Neurofilaments are phosphoproteins. The neurofilaments are one of the five major groups of IFs and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kD, 95kD, and 115kD. Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. |
| Immunogen: | pig spinal cord. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |