Monoclonal Anti-Neurofilament 160 antibody produced in mouse
SIGMA/N5264 - clone NN18, ascites fluid
Synonym: Monoclonal Anti-Neurofilament medium chain
MDL Number: MFCD01322393
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Cucurbitacin E has neuroprotective properties and autophagic modulating activities on dopaminergic neurons. By: Arel-Dubeau, A. M., Longpré, F., et al. in Oxid Med Cell Longev, 2015. PubMed ID: 25574337 Image collected and cropped by CiteAb from the following publication, (http://www.hindawi.com/journals/omcl/2014/425496/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin fixed, paraffin-embedded Rat cerebellum tissue sections were stained with 1:40 Monoclonal Anti-Neurofilament 160 antibody produced in Mouse, Clone: NN-18 (Cat. No. N5264) followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
Western Blotting Whole extract of N1022-Bovine spinal cord neurofilaments were separated on SDS-PAGE and probed with Monoclonal Anti-Neurofilament 160 antibody produced in Mouse, Clone: NN-18 (Cat. No. N5264). The antibody was developed using 1:7,500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:1,000 antibody 2. 1:500 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NN18, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 160 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified, unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue sections |
| immunohistochemistry (frozen sections): suitable | |
| western blot: suitable | |
| UniProt accession no. | P07197  |
| Application: | Monoclonal anti-neurofilament 160 antibody (diluted 1: 5000) can be used as internal loading controls in immunoblotting. It can also be used in whole-mount immunohistochemistry and western blot. Monoclonal anti-neurofilament 160 antibodies can be used for localization of neurofilaments of molecular weight 160,000 in cultured cells or tissues preparation. |
| Application: | Monoclonal anti-neurofilament 160 antibody has been used in • Immunoblotting. • Immunohistochemistry • Immunofluorescence |
| Biochem/physiol Actions: | Mouse monoclonal anti-neurofilament 160 antibody reacts specifically with neurofilaments of molecular weight 160,000 but does not cross react with other intermediate filament proteins. |
| Biochem/physiol Actions: | Neurofilaments play a role in axonal calibre as they help in movement of an impulse down the axon. Their activity depends on phosphorylation of neurofilaments.Mutations of neurofilaments causes Charcot-Marie-Tooth (CMT) disease. Accumulation of neurofilaments has been observed in many human neurological diseases like Alzheimer′s disease, progressive supranuclear palsy, diabetic neuropathy, and giant axonal neuropathy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Neurofilament 160 (mouse IgG1 iso-type) is produced by the fusion mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are composed of three sub-units- light NFL protein, medium NFM protein, heavy NFH protein. Along with there two other intermediate filaments are present α-internexin and peripherin. Neurofilaments are phosphoproteins. |
| General description: | Neurofilaments belong to the intermediate filament family and are expressed mainly in cells or tissues of neuronal origin. It is crucial for proper radial growth of axons. |
| Immunogen: | neurofilaments from pig spinal cord. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |