Anti-NQO1 (C-terminal) antibody produced in rabbit
SIGMA/N5288 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DIA4; Anti-DT-diaphorase; Anti-Diaphorase 4; Anti-NAD (P)H:Menadione oxidoreductase 1, dioxin-inducible 1; Anti-NAD(P)H dehydrogenase, quinone 1; Anti-NMOR1
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 5 μg/mL Anti-NQO1 (C-terminal) antibody produced in Rabbit (Cat. No. N5288) followed by 1:500 Anti-Rabbit IgG-Cy3. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of NIH-3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-NQO1 (C-terminal) (Cat. No. N5288). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 0.2 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.5 μg/mL antibody + NQO1 immunizing peptide (human, 261-274)
Immunoblotting Whole extract of NRK cells was separated on SDS-PAGE and probed with Rabbit Anti-NQO1 (C-terminal) (Cat. No. N5288). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 0.1 μg/mL antibody 2. 0.2 μg/mL antibody 3. 0.5 μg/mL antibody 4. 0.5 μg/mL antibody + NQO1 immunizing peptide (human, 261-274)
Immunoprecipitation Rabbit Anti-NQO1 (C-terminal) (Cat. No. N5288), was used to immunoprecipitate NQO1 (C-terminal) from HeLa whole cell extract. Detection antibody: Rabbit Anti-NQO1. Lanes 1. 2 μg antibody 2. 5 μg antibody 3. Negative control: 2 μg non-relevant antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~28 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg using whole extract of human HeLa cells |
| indirect immunofluorescence: 2-5 μg/mL using human HeLa cells | |
| western blot: 0.1-0.2 μg/mL using whole extract of rat NRK cells | |
| western blot: 0.2-0.5 μg/mL using whole extract of mouse 3T3 cells | |
| UniProt accession no. | P15559  |
| Application: | Anti-NQO1 (C-terminal) antibody may be used in immunological applications such as immunoprecipitation, immunofluorescence and immunoblotting (approximately 28 kDa). |
| Application: | Anti-NQO1 (C-terminal) antibody produced in rabbit has been used in: • western blotting • immunoprecipitation • immunofluorescence |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Anti-NQO1 (C-terminal) antibody is specific for rat, mouse, and human NQO1. The immunizing peptide specifically inhibits the detection of NQO1 band by immunoblotting. |
| Biochem/physiol Actions: | NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) can bioactivate certain antitumor quinones. NQO1 has a role in antioxidant defense via the generation of antioxidant forms of ubiquinone and vitamin E. High levels of NQO1 gene expression has been observed in liver and lung tumors as compared to normal tissues of the same origin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NADPH:quinone oxidoreductase (NQO1) is an FAD containing electron reductase that regulates the detoxification of quinones obtained from the phenolic metabolites of benzene. It is a homodimeric protein that can bind either of the reducing cofactors; NADPH or NADH . NQO1 regulates chemoprotection, neuroprotection, antitumor quinones and antioxidative defense mechanisms . NQO1 may be overexpressed in breast, colon, pulmonary and hepatic tumors. |
| General description: | NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) is a 2-electron reductase. NQO1 is a FAD-containing protein that exists as a homodimer. It is ubiquitously expressed. |
| Immunogen: | synthetic peptide corresponding to amino acid resudues 261-274 of human NQO1 with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat. |
| Immunogen: | Synthetic peptide sequence corresponding to amino acid residues 261-274 of human NQO1, with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |