Monoclonal Anti-Neurofilament 200 antibody produced in mouse
SIGMA/N5389 - clone NE14, ascites fluid
Synonym: NF200 Antibody - Monoclonal Anti-Neurofilament 200 antibody produced in mouse; Neurofilament Antibody; Nf200 Antibody; Monoclonal Anti-Neurofilament heavy chain
MDL Number: MFCD02263444
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Formalin-fixed, paraffin-embedded Rat cerebellum tissue sections were stained with 1:80 Anti-Neurofilament 200 antibody produced in Rabbit (Cat. No. N4142) (A). The antibody was developed using 1:50 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887), and 1:40 Monoclonal Anti-Neurofilament 200 antibody produced in Mouse, Clone: NE14 (Cat. No. N5389) (B). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262). Results Two Anti-Neurofilament 200 antibodies, N4142 (A) and N5389 (B) target different regions of Neurofilament 200 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin-fixed, paraffin-embedded rat cerebellum sections stained with 1:40 Monoclonal Anti-Neurofilament 200 antibody produced in Mouse clone: NE14 (Cat. No. N5389) followed by Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NE14, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide as preservative |
| enhanced validation | independent ( Antibodies) Learn more about Antibody Enhanced Validation  |
| isotype | IgG1 |
| mol wt | antigen apparent mol wt 200 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | pig, mouse, chicken, bovine, guinea pig, rabbit, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 |
| immunohistochemistry (frozen sections): suitable | |
| microarray: suitable | |
| western blot: suitable | |
| UniProt accession no. | P12036  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) Immunohistochemistry (1 paper) |
| Application: | Monoclonal Anti-Neurofilament 200 antibody produced in mouse has been used in • immunohistochemistry • immunolabeling • immunofluorescence |
| Biochem/physiol Actions: | Neurofilament heavy chain (NEFH) gene was recently identified to cause autosomal dominant axonal Charcot-Marie-Tooth disease (CMT2cc). This gene plays a role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Neurofilament 200 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The neurofilaments are one of the five major groups of intermediate filaments (IFs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kD, 160 kD, and 200 kD and are named as NEFL (light), NEFM (medium) and NEFH (heavy) respectively. Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. |
| Immunogen: | pig spinal cord. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |