Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in mouse
SIGMA/N5408 - clone ME20.4, ascites fluid
Synonym: Anti-NGFR p75
MDL Number: MFCD00282384
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:1,000 Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in Mouse, Clone: ME20.4 (Cat. No. N5408) followed by 10 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886) .
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ME20.4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 75 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | mouse, chicken, rat |
| species reactivity | racoon, monkey, pig, human, feline |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tongue | |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| neutralization: suitable | |
| radioimmunoassay: suitable | |
| western blot: suitable | |
| UniProt accession no. | P08138  |
| Application: | Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in mouse has been used in immunoblotting. to added in CB. |
| Application: | Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) may be used: • for enzyme linked immune sorbent assay (ELISA) • immunoprecipitation, immunoblotting and immunohistochemistry • for immunocytochemistry (acetone- or formalin-fixed, Bouin′s solution - or formalin- fixed paraffin-embedded) • for electron- microscopy radioimmunoassay (RIA) (Kd of 7x10-10 M) • flow cytometry • for the inhibition of binding of NGF to the receptor • for rossette assay • as a cholinergic immunotoxin |
| Biochem/physiol Actions: | Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) recognizes the low affinity NGFR p75 of human origin (75 kDa), also known as gp75NGFR, p75NGFR and p75NTR .The epitope recognized by the antibody is located within the third or fourth cysteine-rich repeats of the NGFR molecule. Cross-reactivity has been observed with human, monkey (marmoset), pig, cat and raccoon , but not with rat, chicken or mouse NGFR. |
| Biochem/physiol Actions: | Nerve Growth Factor Receptor (NGFR) p75 enhances the NGF binding affinity of the proto-oncogene product p140trk and also may modulate the kinase activity of p140trk and play a role in signal transduction. Specifically, neurotrophin binding to NGFR p75 stimulates generation of ceramide, activation and translocation of nuclear factor- κ (NF-κB) to the nucleus, and enhancement of Jun kinase (JNK) activity. |
| Biochem/physiol Actions: | Neurotrophins (NT) are growth factors that are essential for the development and maintenance of the nervous system. One of the receptors that mediate the effects of NTs is the Nerve Growth Factor Receptor/TNFRSF16 (p75NGFR, p75NTR). NGFR mediates the effects of NGF that are important in the survival and differentiation of neurons and neural crest-derived cells, during embryonic and early postnatal development. Expression of NGFR has been associated with favorable prognosis and survival in breast and pancreatic cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Nerve Growth Factor Receptor (NGFR p75) (mouse IgG1 isotype) is derived from the ME20.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a human melanoma cell line WM245. The human NGFR p75 has a hydrophobic signal sequence, a single N-linked glycosylation site, four cysteine-rich repeat units of approximately 40 amino acids in the extracellular domain, a serine- and threonine-rich region which might be O-linked glycosylated, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. |
| General description: | Recognizes the low affinity human NGFR p75 (also known as gp75NGFR , p75NGFR and p75NTR). The epitope recognized by the antibody exists within the third or fourth cysteine-rich repeats of the NGFR molecule. |
| Immunogen: | human melanoma cell line WM245. |
| Physical form: | The product is supplied as ascites fluid with 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |