Anti-Nestin antibody produced in rabbit
SIGMA/N5413 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-FJ21841; Anti-NES; Anti-Nbla00170
MDL Number: MFCD03455626
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation. By: Sarrach, S., Huang, Y., et al. in Sci Rep, 2018. PubMed ID: 29379062 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-018-20131-8), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation. By: Sarrach, S., Huang, Y., et al. in Sci Rep, 2018. PubMed ID: 29379062 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-018-20131-8), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat brain sections with 0.05 μg/mL Anti-Nestin antibody produced in rabbit (Cat. No. N5413). The antibody was developed using goat-Anti-Rabbit IgG, DyLight 488 conjugate. (The picture is courtesy of Fabrice GANKAM MD, Ph.D., Néphrologie Dialyse et Transplantation, Hopital Erasme - ULB)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~110 kDa (cleaved) |
| antigen ~177 kDa | |
| antigen ~270 kDa | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 1:200-1:500 |
| immunohistochemistry: 1:100-1:200 | |
| western blot: 1:500-1:1,000 | |
| UniProt accession no. | P48681  |
| Biochem/physiol Actions: | Nestin (NES) functions as a regulator of proteins which are involved in cell growth. It also interacts with other proteins of the intermediate filament. NES has been studied as a marker of neural stem cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Nestin (NES) is a class VI cytoplasmic protein. It is expressed in the intermediate filaments and has a molecular weight of 177kDa. The gene encoding it is localized on human chromosome 1q23.1 with four exons. |
| Immunogen: | synthetic peptide corresponding to residues 254-270 of human Nestin. This sequence is 93% identical in rat. |
| Physical form: | solution in phosphate buffered saline, containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |