Nerve Growth Factor-ß human
SIGMA/N5415 - recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym: β-NGF; BNGF; NGF-β; NGF-beta
Product Type: Chemical
Purity
The purity of human, recombinant Nerve Growth Factor-β expressed in HEK 293 cells (Cat. No. N5415) was assessed on SDS-PAGE under reducing conditions (+DTT) followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result.)
Lanes
1. 0.5 μg/lane
2. 1.25 μg/lane
The purity of human, recombinant Nerve Growth Factor-β expressed in HEK 293 cells (Cat. No. N5415) was assessed on SDS-PAGE under reducing conditions (+DTT) followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result.)
Lanes
1. 0.5 μg/lane
2. 1.25 μg/lane
Purity
The purity of human, recombinant Nerve Growth Factor-β expressed in HEK 293 cells (Cat. No. N5415) was assessed on SDS-PAGE (1 μg/lane) followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result.)
Lane +DTT: Reduced
Lane -DTT: Non-reduced
The purity of human, recombinant Nerve Growth Factor-β expressed in HEK 293 cells (Cat. No. N5415) was assessed on SDS-PAGE (1 μg/lane) followed by InstantBlue™ staining (Cat. No. ISB1L). (Representative result.)
Lane +DTT: Reduced
Lane -DTT: Non-reduced
Bioassay The biological activity of recombinant Nerve Growth Factor-β expressed in HEK 293 cells (Cat. No. N5415) was measured in a cell proliferation assay using TF1 cells. The EC50 is defined as the effective concentration that elicits a 50% increase in cell growth in a cell-based bioassay. (Representative result.)
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/μg Endotoxin (β-NGF) |
| mol wt | protein calculated mol wt 13.5 kDa (Monomer) |
| packaging | pkg of 10 μg |
| pkg of 100 μg | |
| potency | ≤16 ng/mL ED50/EC50 |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01138  |
| Analysis Note: | The specific activity is determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line). |
| Biochem/physiol Actions: | A mutation in the NGF-β gene is implicated in the loss of pain perception. |
| Biochem/physiol Actions: | Nerve growth factor β (β-NGF) is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro. |
| Disclaimer: | The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at -20 °C. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NGF-β gene is mapped to human chromosome 1p13.2. |
| General description: | Recombinant human β-NGF is expressed in human 293 cells as a homodimer (non-disulfide bonded) of 120 amino acids, with a calculated molecular weight of 13.5 kDa (monomer). Nerve growth factor β (β-NGF) is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The production of natural β-NGF occurs in epithelial cells, and the pituitary gland. |
| Physical form: | This product is lyophilized from PBS. |
| Preparation Note: | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile DDW containing 0.1% endotoxin-free recombinant human serum albumin, to a final concentration of 100 μg/ml. |
| Preparation Note: | Store product at –20 °C. The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at –20 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |