Nuclease S1 from Aspergillus oryzae
SIGMA/N5661 - for single-strand DNA/RNA digestion
Synonym: Endonuclease S1
CAS Number: 37288-25-8
MDL Number: MFCD00131010
Product Type: Chemical
Catalyzes the nonspecific endo- and exonucleolytic cleavage of single-stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert super-helical DNA to the linear form.
| application(s) | cell analysis |
| biological source | Aspergillus sp. (A. oryzae) |
| concentration | ≥100000 units/mL |
| form | solution |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| suitability | suitable for nucleic acid purification |
| technique(s) | DNA purification: suitable |
| Application: | Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA. It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor. |
| Biochem/physiol Actions: | Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form. |
| Biochem/physiol Actions: | SI nuclease from Aspergillus oryzae can generate double-stranded DNA breaks in response to DNA nicks or abasic sites. |
| General description: | The Nuclease S1 enzyme from Aspergillus oryzae has the ability to degrade single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides. |
| Other Notes: | One unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C. |
| Physical form: | Solution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl2, 50% glycerol, 2 mg/ml protein |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.30.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |
| Components | 30mM Sodium acetate .25-.25 %; 50mM Sodium chloride .29 %; 1mM Zinc chloride .01 %; Glycerol 50 %; 2mg/mL Protein .2 % |