Neutralizing Buffer
SIGMA/N6019 - for Northern and Southern blotting, powder
Synonym: Neutralizing Buffer for Blotting Techniques; Neutralizing Buffer for Blotting Techniques
Product Type: Chemical
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| foreign activity | DNase, RNase, none detected |
| form | powder |
| grade | Molecular Biology |
| Quality Level | 200  |
| storage temp. | room temp |
| technique(s) | Southern blotting: suitable |
| Application: | Suitable for neutralizing the gel before transfer to membranes during Southern procedures. |
| General description: | Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes. |
| Preparation Note: | The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |