Neomycin trisulfate salt hydrate
SIGMA/N6386 - powder, BioReagent, suitable for cell culture
Synonym: Antibiotic 10676; Neomycin B
CAS Number: 1405-10-3
Empirical Formula (Hill Notation): C23H46N6O13 · 3H2SO4 · xH2O
Molecular Weight: 908.88 (anhydrous basis)
EC Number: 215-773-1
Linear Formula: C23H46N6O13 · 3H2SO4 · xH2O
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N6386-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: N6386-5G
| antibiotic activity spectrum | Gram-negative bacteria |
| Gram-positive bacteria | |
| biological source | Streptomyces sp. |
| color | white to yellow |
| form | powder |
| InChI | 1S/C23H46N6O13.3H2O4S.H2O |
| InChI key | WHAGUNPVKDUVFV-QGTTWHFQSA |
| mode of action | protein synthesis | interferes |
| packaging | pkg of 100 g |
| pkg of 25 g | |
| pkg of 5 g | |
| potency | ≥600 μg neomycin per mg (Dried basis) |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | O.OS(O)(=O)=O.OS(O)(=O)=O |
| solubility | H2O: 50 mg/mL |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Neomycin trisulfate salt hydrate has been used: • to determine Netrin1- encoding gene (Ntn1) protective effects against aminoglycoside antibiotics in cochlear explants • for Escherichia coli antibiotic susceptibility test • in bone marrow transplantation of mice |
| Biochem/physiol Actions: | Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons. Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria. |
| Biochem/physiol Actions: | Neomycin Trisulfateis an aminoglycoside antibiotic, which is produced by Streptomyces containing a minimum of 85% neomycin B.It is used to study the cytotoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interact |
| Disclaimer: | Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture. |
| General description: | As a stock solution. Stock solutions should be filter sterilized and stored at 2-8°C. Stable at 37°C for 5 days. |
| General description: | Chemical structure: aminoglycoside |
| Other Notes: | Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place. |
| Packaging: | 5, 25, 100 g in glass bottle |
| Preparation Note: | Neomycin sulfate is soluble in H2O at 50 mg/mL, yielding a clear solution. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 |
| Hazard Codes | Xi |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |