Anti-NR4A2 (N-terminal) antibody produced in rabbit
SIGMA/N6413 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-HZF-3; Anti-NOT; Anti-NURR1; Anti-RNR1; Anti-TINUR
Product Type: Chemical
Immunofluorescence Cytoplasmic and nuclear staining of NR4A2 in NIH-3T3 cells. Fixed cells were stained with 4 μg/mL Rabbit Anti-NR4A2 (N-terminal) (Cat. No. N6413) followed by Goat Anti-Rabbit IgG, FITC conjugate (x40 magnification).
Immunoblotting Cell lysates of HS68 (Lane 1), HEK293-T non transfected (Lane 2), and HEK-293T overexpressing human NR4A2 (Lanes 3-5) were separated on SDS-PAGE, blotted with Anti-NR4A2 (N-terminal) (Cat. No. N6413) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody 1.5 μg/mL 2. Antibody 1.5 μg/mL 3. Antibody 1.5 μg/mL 4. Antibody 0.75 μg/mL 5. Antibody 1.5 μg/mL + NR4A2 immunizing peptide (human, 14-31)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | ~70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 4-8 μg/mL using 3T3 cells |
| western blot: 1-2 μg/mL using HEK-293T cell lysate expressing human NR4A2 | |
| UniProt accession no. | P43354  |
| Application: | Anti-NR4A2 (N-terminal) antibody has been used in • immunoblotting • immunofluorescence • western blotting |
| Biochem/physiol Actions: | NR4A2 binds the NGFI-B (nerve growth factor-induced clone B) response element (NBRE) sequence (AAAGGTCA) as monomer. NR4A2 is induced by multiple extracellular signals, including fatty acids, stress, growth factors and neurotransmitters in a cell type specific manner. Hepatic expression of NR4A2 is induced by cAMP in response to glucagon and fasting. NR4A2 is essential for the differentiation of nigral dopaminergic neurons. NR4A2 mutations have been associated with disorders related to dopaminergic dysfunction, including Parkinson′s disease, schizophrenia. |
| Biochem/physiol Actions: | Nuclear receptor subfamily 4 group A member 2 (NR4A2) acts as an activator of tyrosine hydroxylase. It is crucial for the process of DNA repair by melanocortin-1 receptor. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NR4A2 (N-terminal) encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, includ |
| General description: | Nuclear receptor subfamily 4 group A member 2 (NR4A2) is part of transcriptional regulator subfamily, under the family of nuclear hormone receptors. It is a transcription factor expressed in the brain and T-cells. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |