Neuraminidase from Vibrio cholerae
SIGMA/N6514 - Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)
Synonym: Acyl-neuraminyl Hydrolase; Receptor-destroying enzyme; Sialidase
CAS Number: 9001-67-6
EC Number: 232-624-6
MDL Number: MFCD00131711
Product Type: Chemical
Neuraminidase non-specific cleavage for sialic acid — α(2-3), α(2-6) and α(2-8) glycosiduic linkages with a variety of natural and synthetic substrates
| biological source | Vibrio cholerae |
| foreign activity | Protease and NAN-aldolase, present |
| form | buffered aqueous solution |
| Quality Level | 200  |
| specific activity | 8-24 units/mg protein (Lowry, using NAN-lactose) |
| storage temp. | 2-8°C |
| type | Type II |
| Analysis Note: | Preservative free. |
| Application: | Neuraminidase from Vibrio cholera has been used in a study to describe a five-step purification method. It has also been used in a study to investigate modification of leukemia L1210 tumor cells. |
| Application: | Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies. |
| General description: | Calorimetry studies show that the two lectin-like domains flanking the central catalytic domain of Neuraminidase serve as the recognition and binding sites for sialic acid-containing substrates. |
| General description: | Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread. |
| Other Notes: | One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate. |
| Physical form: | Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride, 0.2 μm filtered.. |
| Preparation Note: | A further purification by affinity chromatography of our Type III. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 8-24 units/mg protein (Lowry, using NAN-lactose) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.18 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |