Anti-Notch1 antibody, Mouse monoclonal
SIGMA/N6786 - clone mN1A, purified from hybridoma cell culture
Synonym: Notch antibody
MDL Number: MFCD03455659
Product Type: Chemical
Immunoblotting Whole extract of HEK-293T cells overexpressing truncated mouse Notch1 (the intracellular Notch1 molecule -65 kDa) was separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-Notch1 Clone: mN1A (Cat. No. N6786). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Immunoblotting Extract of newborn rat brain was separated on SDS-PAGE and probed with Monoclonal Anti-Notch1 Clone: mN1A (Cat. No. N6786). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. Negative control: without first antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | mN1A, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using extracts of cells stably transfected with a truncated Notch1 (32D-mN1-ICDOP that expresses the intracellular mouse Notch1 molecule-65 kDa). | |
| UniProt accession no. | P46531  |
| Application: | Anti-Notch1 antibody, mouse monoclonal has been used in: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunocytochemistry • immunohistochemistry |
| Biochem/physiol Actions: | NOTCH is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism. Fibronectin functions both as a regulator of cellular processes and as an important scaffolding protein to maintain and direct tissue organization and ECM composition.Fibronectin is suggested to enhance cell adhesion, spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose most of their surface bound fibronectin. |
| Biochem/physiol Actions: | Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch. |
| General description: | The Notch protein family is a group of highly conserved proteins important in the determination of cell fate and maintenance of progenitors in many developmental systems.In mammals, four Notch genes were identified (Notch1-4) in a wide variety of cells and play a crucial role in differentiation and development. Notch proteins contain three main domains: an extracellular domain containing tandem epidermal growth factor repeats, and three lin-12/Notch repeats, a transmembrane domain, and an intracellular domainthat contains six cdc1-/SW16/ankyrin repeats, nuclear localization signals, and a C-terminal OPA/PEST region. |
| Immunogen: | recombinant protein containing the cdc10-NCR region of mouse Notch1. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |