Anti-Neuroglobin antibody produced in rabbit

SIGMA/N7162 - ~1.2 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Ngb

MDL Number: MFCD09265363
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-N7162-200UL	200 µL	$522.00 1/EAAdd To Favorites	Add To Favorites

Immunohistochemistry JOURNAL CITATION: The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats. By: Yang, F., Shan, Y., et al. in Front Neurosci, 2019. PubMed ID: 31191229 Image collected and cropped by CiteAb from the following publication, (https://www.frontiersin.org/article/10.3389/fnins.2019.00537/full), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

antibody form	affinity isolated antibody
antibody product type	primary antibodies
biological source	rabbit
clone	polyclonal
concentration	~1.2 mg/mL
conjugate	unconjugated
form	buffered aqueous solution
Quality Level	200
shipped in	dry ice
species reactivity	rat, human, mouse
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5.0 μg/mL using biotin/ExtrAvidin^®-Peroxidase staining of heat-retrieved rat brain sections
	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5.0-10.0 μg/mL using biotin/ExtrAvidin^®-Peroxidase staining of heat-retrieved human and mouse brain sections
UniProt accession no.	Q9NPG2

Application:	Anti-Neuroglobin antibody produced in rabbit has been used in • immunofluorescence double staining • immunostaining • immunohistochemistry
Biochem/physiol Actions:	Neuroglobin may play an essential role in oxygen homeostasis of neuronal cells. Neuroglobin is thought to protect neurons from hypoxic-ischemic injury as suggested by its increased expression under hypoxic conditions in- vitro as well as in focal cerebral ischemia in vivo. Neuronal survival after hypoxia decreased when neuroglobin expression is inhibited by antisense oligonucleotide and enhanced by neuroglobin overexpression. Its distribution correlates with the subcellular localization of mitochondria and with oxygen demands, possibly linking neuroglobin to cellular respiration.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	Neuroglobin is an oxygen-binding heme protein predominantly expressed in the nervous system in man and other vertebrates. Neuroglobin belongs to the globin superfamily. It displays the typical globin fold, but it shares little amino acid sequence similarity with vertebrate hemoglobins and myoglobins. Neuroglobin is highly conserved among vertebrates and is evolutionary related to the invertebrate nerve globins. In mouse and rat, neuroglobin is preferentially expressed in neuronal cells of the central and peripheral nervous systems, but also in some endocrine tissues. Neuroglobin is highly expressed in neurons of mammalian retina.
Immunogen:	synthetic peptide corresponding to amino acids 55-70 of human neuroglobin, conjugated to KLH.
Legal Information:	ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form:	Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Target description:	Neuroglobin is an oxygen-binding heme proteinpredominantly expressed in the nervous system.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352203