Anti-Neuroglobin antibody produced in rabbit
SIGMA/N7162 - ~1.2 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Ngb
MDL Number: MFCD09265363
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats. By: Yang, F., Shan, Y., et al. in Front Neurosci, 2019. PubMed ID: 31191229 Image collected and cropped by CiteAb from the following publication, (https://www.frontiersin.org/article/10.3389/fnins.2019.00537/full), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats. By: Yang, F., Shan, Y., et al. in Front Neurosci, 2019. PubMed ID: 31191229 Image collected and cropped by CiteAb from the following publication, (https://www.frontiersin.org/article/10.3389/fnins.2019.00537/full), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats. By: Yang, F., Shan, Y., et al. in Front Neurosci, 2019. PubMed ID: 31191229 Image collected and cropped by CiteAb from the following publication, (https://www.frontiersin.org/article/10.3389/fnins.2019.00537/full), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Mouse brain sections were immunostained with 5 mg/mL Anti-Neuroglobin antibody (Cat. No. N7162). Panel A: Cortex Panel B: Hippocampus and Gyrus dentatus Panel C: Nucleus nervi facialis (brain stem) Panel D: Cerebellum including Purkinje cells. The pictures are courtesy of Thomas Hankeln and Tina Hinkelmann (Inst. Molecular Genetics, Univ. Mainz, Germany), and Stefan Reuss (Dept. Anatomy, Univ. Mainz, Germany).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5.0 μg/mL using biotin/ExtrAvidin®-Peroxidase staining of heat-retrieved rat brain sections |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5.0-10.0 μg/mL using biotin/ExtrAvidin®-Peroxidase staining of heat-retrieved human and mouse brain sections | |
| UniProt accession no. | Q9NPG2  |
| Application: | Anti-Neuroglobin antibody produced in rabbit has been used in • immunofluorescence double staining • immunostaining • immunohistochemistry |
| Biochem/physiol Actions: | Neuroglobin may play an essential role in oxygen homeostasis of neuronal cells. Neuroglobin is thought to protect neurons from hypoxic-ischemic injury as suggested by its increased expression under hypoxic conditions in- vitro as well as in focal cerebral ischemia in vivo. Neuronal survival after hypoxia decreased when neuroglobin expression is inhibited by antisense oligonucleotide and enhanced by neuroglobin overexpression. Its distribution correlates with the subcellular localization of mitochondria and with oxygen demands, possibly linking neuroglobin to cellular respiration. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Neuroglobin is an oxygen-binding heme protein predominantly expressed in the nervous system in man and other vertebrates. Neuroglobin belongs to the globin superfamily. It displays the typical globin fold, but it shares little amino acid sequence similarity with vertebrate hemoglobins and myoglobins. Neuroglobin is highly conserved among vertebrates and is evolutionary related to the invertebrate nerve globins. In mouse and rat, neuroglobin is preferentially expressed in neuronal cells of the central and peripheral nervous systems, but also in some endocrine tissues. Neuroglobin is highly expressed in neurons of mammalian retina. |
| General description: | Neuroglobin is an oxygen-binding heme proteinpredominantly expressed in the nervous system. |
| Immunogen: | synthetic peptide corresponding to amino acids 55-70 of human neuroglobin, conjugated to KLH. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |