Anti-Nitric Oxide Synthase, Brain (1409-1429) antibody produced in rabbit
SIGMA/N7280 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-bNOS
MDL Number: MFCD01092426
Product Type: Chemical
Immunohistochemistry Rat cortex tissue stained with Rabbit Anti-Nitric Oxide Synthase, Brain (1409-1429) (Cat. No. N7280). From Dr. Shai Shoham, Research Laboratory, Sarah Herzog Memorial Hospital, Jerusalem, Israel
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Nitric Oxide Synthase, Brain (1409-1429) antibody produced in Rabbit (Cat. No. N7280). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:10,000 antibody 2. 1:20,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 150-160 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:10,000 using rat brain extract |
| UniProt accession no. | P29476  |
| Application: | Anti-Nitric Oxide Synthase, Brain (1409-1429) antibody produced in rabbit has been used in: • immunoblotting • immunostaining • immunohistochemistry • immunofluorescence • immunocytochemistry |
| Biochem/physiol Actions: | Nitric oxide synthase (NOS), brain (nNOS or bNOS) mediates long-term regulation of synaptic transmission (long-term potentiation, long-term inhibition). It catalyzes the production of nitric oxide (NO) from |
| Biochem/physiol Actions: | The antibody is specific for nitric oxide synthase (NOS) derived from brain. It does not recognize NOS derived from activated macrophages (iNOS) and endothelial cells (eNOS). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | In humans, nitric oxide synthase 1 (NOS1) or neuronal nitric acid synthase (nNOS) is localized to the sarcoplasmic reticulum. The gene encoding it is, localized on human chromosome 12q24.22. In rats, the protein is present in perivascular nerves supplying the cerebral vasculature. The gene encoding it is localized on rat chromosome 12q16. |
| General description: | The nitric oxide synthase (NOS) isoform found in neurons is a 150-160 kDa protein. It is also termed brain NOS, NOS1, neuronal NOS (nNOS or bNOS, neuronal constitutive NOS or Ca2+-regulated NOS (cNOS, ncNOS). The nNOS or NOS1 gene is mapped to human chromosome 12q24.22. bNOS is present in skeletal muscle, central, peripheral neurons and neuronal cell bodies. Human bNOS and endothelial nitric oxide synthase (eNOS) share 52% amino acid identity, and rat and human bNOS share 93% amino acid identity. Structurally NOS exists as homodimers. The N-terminal region comprises heme and calmodulin binding sites. The reduced nicotinamide adenine dinucleotide phosphate (NADPH) and flavins binding regions are localized in C-terminal region. |
| Immunogen: | synthetic peptide corresponding to nitric oxide synthase (NOS) of rat brain origin (bNOS, amino acids 1409-1429) conjugated to KLH. The immunogen sequence differs from the human bNOS by a single amino acid residue. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Preparation Note: | Store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |