Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit
SIGMA/N7782 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-iNOS
MDL Number: MFCD00675308
Product Type: Chemical
Western Blotting JOURNAL CITATION: Heterotrimeric G protein-dependent WNT-5A signaling to ERK1/2 mediates distinct aspects of microglia proinflammatory transformation. By: Halleskog, C., Dijksterhuis, J. P., et al. in J Neuroinflammation, 2012. PubMed ID: 22647544 Image collected and cropped by CiteAb from the following publication, (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-111), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting Whole cell extract of Murine RAW 264.7 macrophages activated with LPS and IFN-γ was separated on SDS-PAGE and blotted with Anti-Nitric Oxide Synthase, Inducible antibody produced in Rabbit (Cat. No. N7782). The antibody was developed with 1:5,000 Goat Anti-Rabbit IgG-Alkaline Phosphatase (Cat. No. A9919). Lanes 1. 1:10,000 antibody 2. 1:20,000 antibody 3. 1:10,000 antibody + inhibition with immunizing peptide 4. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 130 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:10,000 using cells extract of murine RAW 264.7 macrophages activated with LPS and IFN-γ. | |
| UniProt accession no. | P29477  |
| Application: | Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit has been used in immunoblotting and immunohistochemistry. |
| Biochem/physiol Actions: | Anti-Nitric Oxide Synthase, Inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). By immunoblotting of an activated mouse macrophage RAW 264.7 cells extract, the staining of the iNOS band is specifically inhibited by the immunizing peptide. |
| Biochem/physiol Actions: | Inducible nitric oxide synthase (NOS2) produces nitric oxide (NO), which is expected to be lethal to malaria parasites in vitro. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Nitric Oxide Synthase, inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). The NOS isoform characterized in macrophages is a 130 kDa protein, also known as macrophage NOS (macNOS; mNOS), NOS2, or inducible NOS (iNOS). iNOS is an interferon (IFN)-γ-inducible protein. This gene is located on human chromosome 17q11. |
| Immunogen: | synthetic peptide corresponding to amino acids 1126-1144 of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS) conjugated to KLH. The immunogen sequence is highly conserved in rat iNOS and diverges from the human iNOS. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |