Neuraminidase from Vibrio cholerae
SIGMA/N7885 - Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)
Synonym: Acyl-neuraminyl Hydrolase; Receptor-destroying enzyme; Sialidase
CAS Number: 9001-67-6
EC Number: 232-624-6
MDL Number: MFCD00131711
Product Type: Chemical
Neuraminidase non-specific cleavage for sialic acid — α(2-3), α(2-6) and α(2-8) glycosiduic linkages with a variety of natural and synthetic substrates
| application(s) | diagnostic assay manufacturing |
| foreign activity | Protease and NAN-aldolase, present |
| form | buffered aqueous solution |
| mol wt | 83 kDa |
| Quality Level | 300  |
| specific activity | 1-5 units/mg protein (Lowry, using NAN-lactose) |
| storage temp. | 2-8°C |
| type | Type III |
| Analysis Note: | Preservative free. |
| Application: | Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase. |
| Application: | Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies. |
| Biochem/physiol Actions: | Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells. |
| General description: | Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release. |
| Other Notes: | One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate. |
| Packaging: | 1 unit in poly bottle |
| Packaging: | 2 units in poly bottle |
| Physical form: | Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2. |
| Preparation Note: | Chromatographically purified. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 1-5 units/mg protein (Lowry, using NAN-lactose) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.18 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |