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4-Nitrophenyl acetate

SIGMA/N8130 - esterase substrate

Synonym: Acetic acid 4-nitrophenyl ester

CAS Number: 830-03-5
Empirical Formula (Hill Notation): C8H7NO4
Molecular Weight: 181.15
EC Number: 212-593-5
MDL Number: MFCD00007326
Linear Formula: CH3CO2C6H4NO2
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-N8130-5G 5 g
$73.30
1/EA
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45-N8130-10G 10 g
$90.80
1/EA
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45-N8130-25G 25 g
$135.00
1/EA
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45-N8130-100G 100 g
$288.00
1/EA
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assay ≥98% (TLC)
form powder or crystals
InChI 1S/C8H7NO4/c1-6(10)13-8-4-2-7(3-5-8)9(11)12/h2-5H,1H3
InChI key QAUUDNIGJSLPSX-UHFFFAOYSA-N
mp 75-77 °C (lit.)
Quality Level 200 
SMILES string CC(=O)Oc1ccc(cc1)[N+]([O-])=O
storage temp. −20°C
Application: 4-Nitrophenyl acetate has been used:
• to evaluate esterase activity in human embryonic kidney cell line
• in carbonic anhydrase inhibition assay
• to study its conversion to p-nitrophenol (p-NP) by the esterase activity of bovine serum albumin
• as a substrate to determine carboxylesterase (CES) activity in mouse liver cells
• to study the advantages of bioconjugation of cross-linked enzyme aggregates (CLEAs) and magnetic iron oxide nanoparticles (MIONPs) in magnetic CLEAs matrix
General description: Chromogenic esterase substrate.
Packaging: 5, 10, 25, 100 g in poly bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥98% (TLC)
mp 75-77 °C (lit.)
Storage Temp. −20°C
UNSPSC 12352204

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