Monoclonal Anti-NF-κB antibody produced in mouse
SIGMA/N8523 - clone NF-12, ascites fluid
Synonym: Anti-CVID12; Anti-EBP-1; Anti-KBF1; Anti-NF-kB; Anti-NF-kB1; Anti-NF-kappa-B1; Anti-NF-kappaB; Anti-NF-kappabeta; Anti-NFKB-p105; Anti-NFKB-p50; Anti-NFkappaB
MDL Number: MFCD00802846
Product Type: Chemical
Immunoblotting Whole extract of NIH-3T3 cells was separated on SDS-PAGE and probed with Monoclonal Anti-NF-κB Clone: NF-12 (Cat. No. N8523). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1:250 antibody 2. 1:500 antibody 3. 1:1,000 antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NF-12, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 65 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| microarray: suitable | |
| western blot: 1:1,000 using a whole cell extract from a mouse fibroblast cell line | |
| UniProt accession no. | P19838  |
| Application: | Monoclonal Anti-NF-κB antibody produced in mouse has been used in immunohistochemistry and Western blotting. |
| Biochem/physiol Actions: | Nuclear factor kappa-B (NF-κB1) has an ability to affect transcription of κB reporter genes positively or negatively when assayed following transfection. |
| Biochem/physiol Actions: | Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) has a role in hippocampal neurogenesis. It regulates the signaling by nuclear factor κB (NF-κB). Deletion of the gene in mice leads to defects in innate and adaptive immunity. The protein acts as a mediator of apoptotic response to S-phase DNA damage and replication stress. NFKB1 modulates the structural plasticity of cortical neurons. Knockout of the protein in mice has been shown to cause liver apoptosis and embryonic death. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-NF-κB p65 (mouse IgG1 isotype) is derived from the NF-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant, mouse NF-κB p65 fragment. Nuclear factor κB (NF-κB1) is a member of NF-κB/Rel family of transcription factors. |
| General description: | Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) is a subunit of nuclear factor |
| Immunogen: | recombinant mouse NF-κB p65 fragment (C-terminal, 151 amino acids). |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |