Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal
SIGMA/N8786 - clone 414, purified from hybridoma cell culture
MDL Number: MFCD02263844
Product Type: Chemical
Immunoblotting
A HeLa cell extract was separated by SDS-PAGE, blotted with Monoclonal Anti-Nuclear Pore Complex Proteins (Cat. No. N8786) and developed using Goat Anti-Mouse IgG-Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. (Lane A) Antibody 1 μ/mL; (Lane B) Negative control (only secondary antibody).
A HeLa cell extract was separated by SDS-PAGE, blotted with Monoclonal Anti-Nuclear Pore Complex Proteins (Cat. No. N8786) and developed using Goat Anti-Mouse IgG-Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. (Lane A) Antibody 1 μ/mL; (Lane B) Negative control (only secondary antibody).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 414, monoclonal |
| concentration | ~3 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 62-210 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, yeast, Xenopus, human |
| target post-translational modification | unmodified |
| technique(s) | electron microscopy: suitable |
| immunocytochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 2-4 μg/mL using HeLa total cell extract |
| Application: | Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used for immunohistochemistry. |
| Application: | Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used in: • immunoblotting • immunoprecipitation • immunocytochemistry • immunoelectron microscopy • immunofluorescence |
| Biochem/physiol Actions: | Nucleocytoplasmic exchanges occur through nuclear pore complexes (NPCs) that are circular apertures in the nuclear envelope where the inner and the outer nuclear membrane are joined. Through the NPC there is a passive transport of small molecules (ions and water) that are smaller than 9-10 nm. Large molecules such as RNA and proteins have to be actively transported through the NPC in an energy and signal dependent manner. Many proteins interact with members of the NPC complex. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma 414 produced by the fusion of mouse myeloma cells (NS-1 cells) and splenocytes from BALB/c mice immunized with rat liver nuclei extract. The nuclear pore complexes (NPCs) are structurally highly conserved in all eukaryotic cells. They contain 30-40 different proteins resulting in a complex with a molecular weight of ~50 kDa. |
| General description: | Nuclear pore complexes (NPCs) are aqueous channels that impart access to the nucleus and modulate the transport of RNA, proteins and ribosome subunits across the nuclear envelope. Nucleoporins, which are conserved proteins, form a complex network and generate these channels. Each NPC is a large, complex cylindrical structure consisting of around 500 nucleoporins. |
| Immunogen: | rat liver nuclei extract. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352203 |