Monoclonal Anti-NG2 antibody produced in mouse
SIGMA/N8912 - clone 132.38, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Chondroitin Sulfate Proteoglycan Melanoma-associated; Anti-MCSP; Anti-MCSPG; Anti-MEL-CSPG MSK16 Chondroitin Sulfate Proteoglycan NG2
MDL Number: MFCD08705593
Product Type: Chemical
Western Blotting Whole extract of Rat brain neonatal cells were separated on SDS-PAGE and probed with Monoclonal Anti-NG2 antibody produced in Mouse, Clone: 132.38 (Cat. No. N8912). The antibody was developed using 1:3,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 132.38, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1 μg/mL using rat brain neonatal extract | |
| UniProt accession no. | Q00657  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting |
| Biochem/physiol Actions: | Chondroitin sulfate proteoglycan 4 (CSPG4) or nerve/glial antigen 2 (NG2) stimulates the activation of integrins in dorsal root ganglion neurons. It also has a role in stimulating inflammatory cytokine expression in microglia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Chondroitin sulfate proteoglycan 4 (CSPG4) or nerve/glial antigen 2 (NG2) is a growth-inhibitory chondroitin sulfate proteoglycan (CSPG) produced after spinal cord injury. It has a single glycosaminoglycan (GAG) chain. The protein belongs to the transmembrane chondroitin sulfate proteoglycans family. |
| Immunogen: | cell extract of HEK293 cells expressing the D3 domain of rat NG2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |